Enable A refseq mRNA sequence (one example is an entrez sequence file which has accession starting with NM_) will allow the program to thoroughly discover the corrsponding genomic DNA and therefore find suitable exon/intron boundaries. Exon junction span
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Enable Selection for the desk of Closest-Neighbor thermodynamic parameters and for the method of melting temperature calculation. Two distinct tables of thermodynamic parameters are available:
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In contrast to random primers, oligo (dT) primers are not suited to degraded samples but are excellent for developing cDNA libraries and recommended for use with reverse transcriptases (RTs) as Superscript IV RT.
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You can’t get and export brass, powder primers or ingredient bullets or ammunition like a non resident.
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Help If the default "Automatic" location is chosen, the program will quickly select the repeat databases using the following procedures.